ABSTRACT
The aim of this paper is to explore the prevention of rabbit postoperative abdominal cavity adhesion with poly (lactic-co-glycotic acid) (PLGA) membrane and the mechanism of this prevention function. Sixty-six Japanese white rabbits were randomly divided into normal control group, model control group and PLGA membrane group. The rabbits were treated with multifactor methods to establish the postoperative abdominal cavity adhesion models except for those in the normal control group. PLGA membrane was used to cover the wounds of rabbits in the PLGA membrane group and nothing covered the wounds of rabbits in the model control group. The hematologic parameters, liver and kidney functions and fibrinogen contents were detected at different time. The rabbit were sacrificed 1, 2, 4, 6, 12 weeks after the operations, respectively. The adhesions were graded blindly, and Masson staining and immunohistochemistry methods were used to observe the proliferation of collagen fiber and the expression of transforming growth factor β1 (TGF-β1) on the cecal tissues, respectively. The grade of abdominal cavity adhesion showed that the PLGA membrane-treated group was significant lower than that in the model control group, and it has no influence on liver and kidney function and hematologic parameters. But the fibrinogen content and the number of white blood cell in the PLGA membrane group were significant lower than those of model control group 1 week and 2 weeks after operation, respectively. The density of collagen fiber and optical density of TGF-β1 in the PLGA membrane group were significant lower than those of model control group. The results demonstrated that PLGA membrane could be effective in preventing the abdominal adhesions in rabbits, and it was mostly involved in the reducing of fibrinogen exudation, and inhibited the proliferation of collagen fiber and over-expression of TGF-β1.
Subject(s)
Animals , Rabbits , Abdominal Cavity , General Surgery , Collagen , Metabolism , Lactic Acid , Polyglycolic Acid , Tissue Adhesions , Transforming Growth Factor beta1 , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of PLGA absorbable membrane in prevention of postoperative abdominal adhesion in rabbits.</p><p><b>METHODS</b>66 Japanese white rabbits were randomly divided into three groups: the normal control group n = 6, model control group n = 30 and PLGA group n = 30. Rabbits were received multifactor methods to establish postoperative abdominal adhesion models except for normal control group. The cecum wound was covered PLGA membrane in the PLGA group. At postoperative 1, 2, 4, 6 and 12 weeks, the abdominal cavities were reopened and the adhesive severity was graded blindly, and the hydroxyproline level in cecum tissue was measured and the cecum histopathology was observed.</p><p><b>RESULTS</b>(1) the degree of adhesion and hydroxyproline level in model control group were significantly higher than those of normal control group (P < 0.05, P < 0.01), while the degree of adhesion and hydroxyproline level in PLGA group were significantly lower than those of model control group (P < 0.05). (2) HE staining showed that cecum serosa had obviously inflammatory cell infiltration and fibroblast proliferation, while PLGA could inhibit fibroblast proliferation and reduce the inflammatory cell infiltration and collagen.</p><p><b>CONCLUSION</b>PLGA absorbable membrane can inhibit fibroblast proliferation and collagen to prevent the experimental postoperative peritoneal adhesions.</p>
Subject(s)
Animals , Rabbits , Abdominal Cavity , Pathology , Cell Proliferation , Collagen , Fibroblasts , Cell Biology , Lactic Acid , Chemistry , Membranes, Artificial , Polyglycolic Acid , Chemistry , Postoperative Complications , Tissue AdhesionsABSTRACT
@#Objective To investigate the effect of the ultrasonic therapy on the neurological function after cerebral infarction. Methods 223 cases with cerebral infarction were divided into two groups: treatment group and control group. Both groups received the similar treatment with drug, while the patients in treatment group were treated with the ultrasonic therapy. They were assessed with Clinical Neurological Functional Deficit before and after treatment. Results The scores of Neurological Functional Deficit of all patients decreased (P<0.05), and treatment group decreased more significantly than control group (P<0.05). The total effective rate in the treatment group was higher than that in control group (P<0.05). Conclusion The ultrasonic therapy is helpful for the functional recovery of the patients after cerebral infarction.
ABSTRACT
@#Objective To investigate the effect of the ultrasonic therapy on the neurological function after cerebral infarction. Methods 223 cases with cerebral infarction were divided into two groups: treatment group and control group. Both groups received the similar treatment with drug, while the patients in treatment group were treated with the ultrasonic therapy. They were assessed with Clinical Neurological Functional Deficit before and after treatment. Results The scores of Neurological Functional Deficit of all patients decreased (P<0.05), and treatment group decreased more significantly than control group (P<0.05). The total effective rate in the treatment group was higher than that in control group (P<0.05). Conclusion The ultrasonic therapy is helpful for the functional recovery of the patients after cerebral infarction.
ABSTRACT
@#Objective To investigate the effect of the ultrasonic therapy on the neurological function after cerebral infarction. Methods 223 cases with cerebral infarction were divided into two groups: treatment group and control group. Both groups received the similar treatment with drug, while the patients in treatment group were treated with the ultrasonic therapy. They were assessed with Clinical Neurological Functional Deficit before and after treatment. Results The scores of Neurological Functional Deficit of all patients decreased (P<0.05), and treatment group decreased more significantly than control group (P<0.05). The total effective rate in the treatment group was higher than that in control group (P<0.05). Conclusion The ultrasonic therapy is helpful for the functional recovery of the patients after cerebral infarction.
ABSTRACT
@#Objective To investigate the effect of the ultrasonic therapy on the neurological function after cerebral infarction. Methods 223 cases with cerebral infarction were divided into two groups: treatment group and control group. Both groups received the similar treatment with drug, while the patients in treatment group were treated with the ultrasonic therapy. They were assessed with Clinical Neurological Functional Deficit before and after treatment. Results The scores of Neurological Functional Deficit of all patients decreased (P<0.05), and treatment group decreased more significantly than control group (P<0.05). The total effective rate in the treatment group was higher than that in control group (P<0.05). Conclusion The ultrasonic therapy is helpful for the functional recovery of the patients after cerebral infarction.